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In order to investigate the properties of cephalosporinase, a strong antibiotic resistant strain(H 112) was isolated from Pseudomonas aeruginose.
The extracted enzyme had the following characteristics. Optimum temperature was 451C and unstable over 55C, and optimum pH was 8.5. Cephalosporinase activity was not inhibited by metal ions such as Mn´*, Cu**, Fe++ Fe+++ and EDTA. But it was inhibited by some antibiotics such as carbenicillin, cefoxitin, cefotaxime, cefamandole, cefoperazone, and SDS. The Vmax values of the enzyme were 100 at cephaloridine and 2.8 at cefoperazone, respectively. The molecular weight of cephalosporinase was estimated to be about 37.500±3, 000 by high performance liquid chromatography and nitrocefin reaction. Key words: Cephalosporinase, Pseudomonas aeruginosa
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